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Anti-CREB (phospho S133)抗体[E113]
参阅全部 CREB 一抗
兔单克隆抗体[E113] to CREB (phospho S133)
Rabbit
This antibody is specific for CREB phosphorylated on Serine 133. The immunogen of the antibody shares 94%homology with CREB (S136) and 86% homology with ATF1 (pS63). No experiment has been performed to verify the possible cross-reactivity.
适用于: WB, IHC-P, IP, Flow Cyt, Dot blot, ICC/IFmore details
与反应: Mouse, Rat, Human
预测可用于: Chicken, Cow, Zebrafish
Synthetic peptide within Human CREB aa 100-200. The exact sequence is proprietary.
Database link: P16220
(Peptide available as
WB: A431 cell lysate; HeLa treated with anisomycin for 30 minutes ICC/IF: A431 and HeLa IHC-P: Thyroid gland adenocarcinoma. IP: HeLa treated with 25 ug/mL anisomycin for 30 minutes.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information see here.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free production
Our Abpromise guarantee covers the use of ab32096 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
WB | 1/5000. Predicted molecular weight: 37 kDa. For unpurified use at 1/500. | |
IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
IP | 1/40 - 1/100.
| |
Flow Cyt | Use at an assay dependent concentration. | |
Dot blot | 1/1000. | |
ICC/IF | 1/100 - 1/250. |