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Anti-BrdU抗体[IIB5]

货号:ab8152

销售价格: ¥3805(市场价:¥3805) (赠送3805积分) 收藏

品牌: ABCAM

赠送积分: 3805积分

购买数量: - +

商品货号 规格 基本价格
500ul 询价
  • 产品名称

    Anti-BrdU抗体[IIB5]
    参阅全部 BrdU 一抗

  • 描述

    小鼠单克隆抗体[IIB5] to BrdU

  • 宿主

    Mouse

  • 特异性

    BrdU is a thymidine analogue and when offered to proliferating cells it is incroporated into reduplicating cells. The antibody is specific for DNA in which BrdU has been incorporated. In immunoassays this antibody reacts strongly with free or carrier-protein coupled BrdU but not with other nucleosides. In immuncytochemistry the antibody only recognizes BrdU in denaturated (single stranded) DNA. The BrdU antibody is 100% crossreactive with Iodo-Deoxy-Uridine (IrdU). Therfore, IdU instead of BrdU can be used in studies.

  • 经测试应用

    适用于: IHC-FoFrICC/IFFlow CytIHC-FrIHC-Pmore details

  • 种属反应性

    与反应: Species independent

  • 免疫原

    Chemical/ Small Molecule BrdU conjugated to BSA.

  • 阳性对照

    • Bromodeoxyuridine labeled cells.

  • 常规说明

    The following product is available as purified antibody (purified by affinity chromatography) together with several conjugated versions:

    Anti-BrdU antibody [BU1/75 (ICR1)] (ab6326)

    Unstained positive control slides from mice treated with BrdU (formalin-fixed, paraffin-embedded intestine sections) are available as BrdU control slides ab129956.

     

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab8152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用Ab评论说明
IHC-FoFr
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Flow Cyt
1/100 - 1/200.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr
1/5 - 1/10.

Dilute antibody in 0.15 M phosphate buffered saline with 1% BSA and 1% Na-azide.

IHC-P
1/5 - 1/10. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Enzymatic antigen retrieval with proteases can also be used.


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