所有分类
Anti-BrdU抗体[IIB5]
参阅全部 BrdU 一抗
小鼠单克隆抗体[IIB5] to BrdU
Mouse
BrdU is a thymidine analogue and when offered to proliferating cells it is incroporated into reduplicating cells. The antibody is specific for DNA in which BrdU has been incorporated. In immunoassays this antibody reacts strongly with free or carrier-protein coupled BrdU but not with other nucleosides. In immuncytochemistry the antibody only recognizes BrdU in denaturated (single stranded) DNA. The BrdU antibody is 100% crossreactive with Iodo-Deoxy-Uridine (IrdU). Therfore, IdU instead of BrdU can be used in studies.
适用于: IHC-FoFr, ICC/IF, Flow Cyt, IHC-Fr, IHC-Pmore details
与反应: Species independent
Chemical/ Small Molecule BrdU conjugated to BSA.
Bromodeoxyuridine labeled cells.
The following product is available as purified antibody (purified by affinity chromatography) together with several conjugated versions:
Anti-BrdU antibody [BU1/75 (ICR1)] (ab6326)
Unstained positive control slides from mice treated with BrdU (formalin-fixed, paraffin-embedded intestine sections) are available as BrdU control slides ab129956.
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, PBS
浓度
Tissue culture supernatant
单克隆
IIB5
Sp2/0-Ag14
IgG1
Our Abpromise guarantee covers the use of ab8152 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
IHC-FoFr | Use at an assay dependent concentration. | |
ICC/IF | Use at an assay dependent concentration. | |
Flow Cyt | 1/100 - 1/200. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. | |
IHC-Fr | 1/5 - 1/10. Dilute antibody in 0.15 M phosphate buffered saline with 1% BSA and 1% Na-azide. | |
IHC-P | 1/5 - 1/10. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Enzymatic antigen retrieval with proteases can also be used. |